Micromechanical studies of mitotic chromosomes
نویسندگان
چکیده
منابع مشابه
Building mitotic chromosomes
Mitotic chromosomes are the iconic structures into which the genome is packaged to ensure its accurate segregation during mitosis. Although they have appeared on countless journal cover illustrations, there remains no consensus on how the chromatin fiber is packaged during mitosis. In fact, work in recent years has both added to existing controversies and sparked new ones. By contrast, there ha...
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The objective of this study was to determine whether the mitotic factors of HeLa cells, which induce meiotic maturation, i.e. germinal vesicle breakdown (GVBD) and chromosome condensation, when injected into fully grown Xenopus laevis oocytes, were localized in the cytoplasm or associated with the metaphase chromosomes. Cytoplasmic extracts were prepared by lysing mitotic HeLa cells in low-salt...
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In the preceding article we described a polyclonal antibody that recognizes cSc-1, a major polypeptide component of the chicken mitotic chromosome scaffold. This polypeptide was shown to be chicken topoisomerase II. In the experiments described in the present article we use indirect immunofluorescence and immunoelectron microscopy to examine the distribution of topoisomerase II within intact ch...
متن کاملThe bending rigidity of mitotic chromosomes.
The bending rigidities of mitotic chromosomes isolated from cultured N. viridescens (newt) and Xenopus epithelial cells were measured by observing their spontaneous thermal bending fluctuations. When combined with simultaneous measurement of stretching elasticity, these measurements constrain models for higher order mitotic chromosome structure. We measured bending rigidities of B approximately...
متن کاملHigher-order structure of human mitotic chromosomes.
From observations on the partial disintegration of isolated human metaphase chromosomes we propose that human metaphase chromatids have a rather simple organization based on the folding and coiling of a long, regular, hollow cylindrical structure with a diameter of about 4000 A. This cylindrical structure, the unit fiber, is postulated to be a super-solenoid formed by the coiling of a 300 A sol...
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ژورنال
عنوان ژورنال: Chromosome Research
سال: 2008
ISSN: 0967-3849,1573-6849
DOI: 10.1007/s10577-008-1233-7